pT7-6×His-EGFP-Spi1(mouse-opt-ecoli)-6×His

pT7-6×His-Atl2(rat-toxic-opt)-6×His

pT7-clyA(ecoli)-GP2-EGFP

pT7-clyA(ecoli)-E75-EGFP（低拷贝）

pT7-Gsdmd(mouse)-6×His

pT7-MBP-Hs3st1(mouse-opt-ecoli)-6×His

pTac-GST-TRAF1(human)

pT7-6×His-PGRMC1(human)-6×His(低拷贝)

pT7-6×His-GST-USP39(human)

pTac-GST-EGLN1(human)